Information on Groups of Mice

GeneNetwork (GN) contains data from different groups of mice. The BXD genetic reference panel (GRP) will be your best bet if you are just exploring GN. All groups must have a genotype data set (under Type) and most also have a phenotpe data set (also under Type). Phenotype in this case usually means classical trait data that we have gathered from various publications. Molecular expression data, when available, are listed with the suffix "mRNA."

Here are the groups to select for access to particular types of data:

• AKXD: Expression data for mammary tumors generated by Kent Hunter at NCI.
• AXB/BXA: Classical phenotypes that have usualy been collected from the literature.
• B6BTBRF2: Expression data for liver generated by Alan Attie and colleagues.
• B6D2F2: Expression data for whole brain generated by Robert Hitzemann and colleagues.
• BDF2-2005: Expression data for striatum generated by Robert Hitzemann and colleagues. The striatum is a large forebrain region involved in learning and movement control that is severely affected in Huntington's and Parkinson's diseases.
• BXD: Expression data for brain and several brain regions, including the cerebellum, hippocampus, and striatum. BXD expression data for whole eye, liver, and hematopoietic stem cells. Classical phenotypes that have usualy been collected from the literature.
• BHF2: F2 cross of BXH. Expression data for adipose, brain, liver, and muscle tissue generated by Jake Lusis at UCLA and Eric Schadt at Rosetta.
• BXH: Only classical phenotypes that have usually been collected from the literature.
• CXB: Expression data for the hippocampus; a brain region involved in learning and memory, epilepsy, and neurodegenerative diseases. Classical phenotypes have also been collected from the literature.
• Heterogeneous Stock (HS) mice: Three expression data sets for hippocampus, lung, and liver are currently available. Phenotype and genotype data are also available at http://gscan.well.ox.ac.uk/.
• LXS: Phenotypes that have usually been generated by investigators at the Institute of Behavior Genetics and at the University of Tennessee over the last few years. We expect the addition of several large brain expression data sets late in 2006.
• MDP: The great majority of data on the Mouse Diversity Panel is taken from the Phenome Project. Unlike the PHone Project, the MDP also includes limited data from the older literature. If you would like to add phenotypes, please submit your data to RW Williams and colleagues.

The AKXD recombinant inbred (RI) strains are derived from AKR/J (AK) and DBA/2J (D). All of these strains were made by Benjamin A. Taylor at the Jackson Laboratory.

All of the AKXD data in GeneNetwork is from an experiment by Kent Hunter and colleagues. GN does not yet include a Phenotypes database for this strain set.

All strains have been genotyped using the Wellcome-CTC-Illumina set of SNPs (13377), as well as some microsatellites, and other markers. WebQTL exploits a total of 5448 markers that are infomative in this mapping panel (Aug 2005).

There are a total of 1027 known recombinations in the AKXD set; an average of 42.8 recombinations per strain (Shifman et al., 2006).

How to obtain these strains: These strains are now cryopreserved. To rederive these strains please contact the Jackson Laboratory and see http://jaxmice.jax.org/jaxmicedb/html/rcbinbred.shtml.

For more details on the history, generation, and use of RI strains as genetic reference populations for systems genetics please see Silver (1995). Additional useful literature links are provided in the References link at the top center of this page.

The AXB and BXA set of recombinant inbred (RI) strains were derived from a reciprocal cross between A/J (A) and C57BL/6J (B). A particular advantage of this RI set (shared with BXD) is that the two parental strains have both been sequenced and are known to differ at approximately 1.80 million SNPs. Variants (mostly single nucleotide polymorphisms and insertion-deletions) that may produce interesting phenotypes can be located efficiently. The zoomable physical maps in WebQTL display the positions of these A-type versus B-type SNPs down at high resolution.

Data acquired using AXB and BXA subsets should be combined; the only difference being the polarity of intercross matings that generated (A x B)F1s and (B x A)F1s. AXB and BXA strains were all produced by Muriel Nesbitt at UCSD in the mid and late 1970s and first used in the early 1980s (Skamene et al., 1984; Peleg and Nesbitt, 1984; Marshal et al., 1992). The set was imported into The Jackson Laboratory by Beverly Paigen (Pgn) in the early 1990s. As of 2004, approximately 25 viable and fully independent AXB/BXA strains are available. These strains are not segregating for the more recent A/J retrotransposon mutation in the dysferlin gene (Ho et al., 2004).

Approximately 122 traits are currently included in the AXBXA Phenotypes database (July 2005).

All strains have been genotyped using the Wellcome-CTC-Illumina set of SNPs (n = 13377), as well as some microsatellites, and other markers. WebQTL exploits a total of 8514 markers that are infomative in this mapping panel (Aug 2005).

There are a total of approximately 1600 known recombinations (corrected for five "duplicate strains") in the AXB/BXA set; an average of 54.4 recombinations per strain (Shifman et al., 2006).

Several nominally independent strains in the AXB and BXA sets are very closely related. These duplicates should not be used without special statistical precaution. The most obvious option is to combine and average data from these strains except when their phenotypes differ significantly (Taylor 1996; Williams et al., 2001).

AXB13=AXB14: 92.74% identity in an analysis of 8429 markers.
AXB18=AXB19=AXB20: 97 to 99% identity (AXB18 to AXB19 = 98.16% identity, AXB18 to AXB20 = 95.72% identity, AXB19 to AXB20 = 97.34% identity n an analysis of 8429 markers)
BXA8=BXA17: 99.79% identity in an analysis of 8429 markers. (Updated from Williams et al. 2001).

How to obtain these strains: Please see http://jaxmice.jax.org/jaxmicedb/html/rcbinbred.shtml.

For more details on the history, generation, and use of RI strains as genetic reference populations for systems genetics please see Silver (1995). Additional useful literature links are provided in the References link at the top center of this page.

The BXD set of recombinant inbred (RI) strains were derived by crossing C57BL/6J (B6) and DBA/2J (D2) and then inbreeding progeny for many generations. This genetic reference panel is a remarkable resource because data for hundreds of interesting traits have been acquired over a 25-year period. Another advantage of the BXD is that the both parental strains have been sequenced (C57BL/6J as part of a public effort, and DBA/2J by Celera Genomics). Based on our analysis of the sequence data, these two strains differ at approximately 1.75 million SNPs. Variants (mostly single nucleotide polymorphisms and insertion-deletions) that produce interesting phenotypes can be located efficiently. The zoomable physical maps in WebQTL can display the positions of B versus D-type SNPs at high resolution.

BXD1 through BXD32 were produced by Benjamin A. Taylor starting in the late 1970s. BXD33 through BXD42 were also produced by Taylor, but from a second set of crosses initiated in the early 1990s. These strains are all available from the Jackson Laboratory, Bar Harbor, Maine. BXD43 through BXD100 were produced by Lu Lu, Jeremy Peirce, Lee M. Silver, and Robert W. Williams in the late 1990s and early 2000s using advanced intercross progeny (Peirce et al. 2004). These new strains have roughly twice the number of recombinations of conventional F2-derived RI strains. The new BXD strains are available now from Lu Lu and colleagues at the University of Tennessee.

Strain nomenclature: Some of the BXD strains have accumulated new mutations that have recently been characterized. When these mutations are known, the full nomenclature of the strain is now being modified. For example, BXD24/TyJ (aka BXD24 in most GeneNetwork databases), suffered a mutation in the Cep290 gene in the late 1980s. The mutant allele (rd16 is associated with autosomal recessive retinal degeneration. The original BXD strain was briefly referred to as BXD24a/TyJ, while the blind co-isogenic mutant was referred to as BXD24b/TyJ. The great majority of phenotype, expression, and genotype data in GeneNetwork was generated using these blind BXD24b/TyJ animals. However, in 2010, the nomenclature was changed again and the blind variant (JAX stock 000031) is now known as BXD24/TyJ-Cep290rd16/J. The original BXD was rederived from frozen stock and is now known once again as BXD24/TyJ, although the stock number has now been changed to 005243.

BXD29/TyJ was also known as BXD29/TyJ-Tlr4, but is now formally BXD29-Tlr4lps-2J/J (JAX stock 000029). The original non-mutant stock is currently known as BXD29/TyJ again but the stock number of these rederived non-mutants has been changed to 010981.

The mitochondrial DNA of all BXD strains were typed by Jing Gu and Shuhua Qi (Nov 2004) using DNAs obtained from the Jackson Laboratory (BXD1 through 42) or from the UTHSC colony. This typing relied on a SNP marker identified by Jan Jiao in Weikuan Gu's laboratory at nucleotide position 9461 in the reference C57BL/6J mitochondrial sequence. Most strains have inherited mitochondria from C57BL/6J. However, the following strains have mitochondria with a DBA/2J allele at the UT-M-9461 SNP: BXD32, 61, 74, 76, 82, 89, 90, 91, 95, and BXD99. (These ten strains could be considered DXB recombinant inbred strains.) The only surprise in this list is that BXD32/TyJ has a DBA/2J mitochondrial genotype at this position.

Genotypes of these strains: All BXD strains were genotyped in the first half of 2005 at 13377 markers as part of a CTC-Wellcome Trust collaboration. When combined with previous markers, there are a total of 7636 informative markers that differ betweeen the parental strains and that are useful for mapping with the BXD strains. The locations of these makers are known on the latest assembly of the mouse genome (Build 34, mm6). The median distance between these informative markers is 178,831 bp. The mean distance is 324,493 bp. There are only 26 intervals between markers that are longer than 5 Mb. No interval is greater than 10 Mb except on Chr X. These long intervals are essentially monomorphic between the parental strains.

The BXD genotype files used in WebQTL include a selected subset of 3795 markers (out of 7636) that includes all those markers with unique strain distribution patterns (SDP) as well as pairs of markers--the most proximal and most distal--for SDPs represented by two or more markers. This BXD genotype data set can be downloaded by ftp at ftp://atlas.utmem.edu/Public/BXD_WebQTL_Genotypes.

There are a total of 1848 known recombinations in the 36 older (JAX) BXD set; an average of 48.1 recombinations per strain.

There are a total of 4366 known recombinations in the 53 new (UTHSC) BXD set; an average of 82.4 recombinations per strain (Shifman et al., 2006). These RI strains were generated from an advanced intercross, and this accounts for the higher recombination load (Peirce et al., 2005).

Approximately 798 phenotypes are currently included in the BXD Phenotypes database (July 2005).

How to obtain these strains: Please see http://jaxmice.jax.org/strain/000105.html. Cost of the JAX BXD strains is approximately $65.40 each (2008). To obtain strains BXD43 through BXD100 please contact Lu Lu. Approximately half of the new BXD strains are now fully inbred (greater than generation F21).

For more details on the history, generation, and use of RI strains as genetic reference populations for systems genetics please see Silver (1995). Additional useful literature links are provided in the References link at the top center of this page.

Information on array platform GEO GPL2510.

This June 2005 data freeze provides estimate of mRNA expression in (adult) brains of F2 intercross mice (C57BL/6J x C3H/HeJ) on ApoE null backgrouds, measured using Agilent microarray pairs. Data were generated at The Univesity of California Los Angeles (UCLA), by Jake Lusis and Thomas Drake. Data were processed using mlratio method developed by He and colleagues (2003 -- Paper with He and Schadt).

The (C57BL/6J X C3H/HeJ)F2 intercross consists of 334 animals of both sexes. All are ApoE null and received a high fat "Western" diet from 8-24 weeks of age. These have been genotyped for QTL mapping, and various phenotypes measured.

For more information contact Leslie Ingram or Jake Lusis at UCLA.

The BXH set of recombinant inbred strains (RI) were made by crossing female C57BL/6J (B) with male C3H/HeJ (H) mice. Benjamin Taylor created the initial 12 BXH recombinant inbred strains at The Jackson Laboratory in 1969. A second set of eight BXH strains were generated by Linda Siracusa at the Kimmel Cancer Center (Kcc) in 1995. She selected for tyrosinase-negative albinos; a gene on the central part of chromosome (Chr) 7. Four of these new BXH strains (one now relabeled as a recombinant congenic) are now also available from The Jackson Laboratory. The following are the old and new symbols for the four recent additions:

• BXHA1/Sr = BXH20/Kcc
• BXHA2/Sr = BXH21/Kcc
• BXHB2/Sr = BXH22/Kcc
• BXHE1/Sr = B6cC3-1/Kcc (backcrossed to B6 and a recombinant congenic)

Approximately 142 traits are currently included in the BXH Phenotype database (July 2005).

All strains have been genotyped using the Wellcome-CTC-Illumina set of SNPs (13377), as well as some microsatellites, and other markers. WebQTL exploits a total of 8311 markers that are infomative in this mapping panel (Aug 2005).

There are a total of 775 known recombinations in the 16 core BXH strains; an average of 48.4 recombinations per strain (Shifman et al., 2006).

How to obtain these strains: Please see http://jaxmice.jax.org/jaxmicedb/html/rcbinbred.shtml.

For more details on the history, generation, and use of RI strains as genetic reference populations for systems genetics please see Silver (1995). Additional useful literature links are provided in the References link at the top center of this page.

BXH2 is susceptible to M. bovis (tuberculosis) and malaria infections despite Nramp1 resistance due to an Icsbp1 (Irf8) mutation. (P Gros and colleagues).

The CXB set is the first, oldest, and smallest group of mouse recombinant inbred (RI) strains. The maternal strain is BALB/cBy and the paternal strain is C57BL/6By. Donald Bailey, the inventor of recombinant inbred strains, created the initial eight BXH recombinant inbred strains at The Jackson Laboratory in the mid 1960s. They were then called CXB-A through CXB-H. They have been used extensively by immunologists and neurogeneticists. A total of 13 of these strains are now available.

Approximately 506 traits are now included in the CXB Phenotype database (July 2005).

All strains have been genotyped using the Wellcome-CTC-Illumina set of SNPs (13377), as well as some microsatellites, and other markers. WebQTL exploits a total of 1384 markers that are infomative in this mapping panel (Aug 2005).

There are a total of 694 known recombinations in the 13 CXB strains; an average of 53.4 recombinations per strain (Shifman et al., 2006).

How to obtain these strains: Please see http://jaxmice.jax.org/jaxmicedb/html/rcbinbred.shtml.

For more details on the history, generation, and use of RI strains as genetic reference populations for systems genetics please see Silver (1995). Additional useful literature links are provided in the References link at the top center of this page.

The parental strains of the LXS recombinant inbred (RI) set are Inbred Long-Sleep (ILS) and Inbred Short-Sleep (ISS) strains. These parental strains have been phenotyped intensively by behavioral geneticists and neuropharmacologists for a decade. The LXS RI set has an intriguing history and trace back to an 8-way cross initiated in the 1950s by Gerald McClearn, the dean of mouse behavior genetics.

The LXS panel has recently been genotyped at approximately 330 microsatellites (Williams et al., 2005) and at 5000 informative SNPs (Wellcome-CTC consortium). The GeneNetwork uses a subset of 2659 markers to map Mendelian and quantitative trait loci in this large panel.

As of July 2005 approximately 10 cohorts of traits (71 total records) have been entered into GeneNetwork's Phenotypes database.

All of the LXS strains have been genotyped using the Wellcome-CTC-Illumina set of SNPs (13377), as well as some microsatellites, and other markers. WebQTL exploits a total of 5178 markers that are infomative in this mapping panel (Aug 2005).

There are a total of 3598 known recombinations in the 77 LXS strains genotyped by Wellcome-CTC; an average of 46.7 recombinations per strain (Shifman et al., 2006).

For information on the availability of the LXS strains please contact Beth Bennett.

For more details on the history, generation, and use of RI strains as genetic reference populations for systems genetics please see Silver (1995). Additional useful literature links are provided in the References link at the top center of this page.

The Mouse Diversity Panel consists of approximately 122 of the major inbred strains, substrains, congenic strains, and even some common mutant strains of mice used in biomedical research. Full descriptions of most of these strains are available from the Jackson Laboratory, and especially the Phenome Project web site. We have usually listed specific substrains of mice, most with the "/J" suffix.

For information on the correct nomenclature for the 129 strains see http://www.informatics.jax.org/mgihome/nomen/strain_129.shtml

Known nomenclature problems (April 2008)

1. BTBR T+ tf/J is also listed at the bottom of the Trait Data page as BTBRT<+>tf/J. This is a single strain.
2. C57Bl/6ByJ should be listed as C57BL/6ByJ
3. CZECHI/EiJ should probably be CZECHII/EiJ

Fifty-six Filial generation 2 (F2) mice were generated by crossing C57BL/6J (B6) and DBA/2J (D2) stock from the Jackson Laboratory. The F1s were mated reciprocally to create B6D2F2 and D2B6F2 progeny. At present , WebQTL includes one large microarray data set (Affymetrix M430) for the entire brain of these F2 progeny.

For further information, please contact John Belknap, Department of Behavioral Neuroscience, Oregon Health & Science University, Portland VA Medical Center, Portland, OR 97239.

This cross consists of a subset of 60 F2 progeny generated by crossing C57BL/6J and BTBR strains. All of these cases are homozygous for the spontaneous obese mutation in the leptin gene (Lep-ob/ob). Metabolic function, liver mRNA expression (Agilent platform), and other physiological and molecular traits related to type 2 diabetes and obesity were quantified. Liver gene expression data were generated by Hong Lan and Alan Attie at The University of Wisconsin-Madison. Please contact Drs. Alan Attie regarding use of this data set in publications or projects.

    About this file:

The file started, Nov 5, 2004 by RWW. Last update by RWW, Nov 6, Dec 17, 2004; April 10, 2005; July 15, 2005. EJC Mar 23, 2006. RWW July 26, 2006. RWW April, 2008. EGW July, 2008.